Overview

Product Name

Caspase 9 cleaved Asp315 antibody

Product Number

IR100-414

Gene Description

caspase 9, apoptosis-related cysteine peptidase

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Recommended

Applications Dilutions

Western Blot 1:500

Immunofluorescence 1:300 – 1:400

Immunohistochemistry (Paraffin) 1:100 – 1:300

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.17 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

IR100-414 anti-Caspase 9 cleaved Asp315 antibody WB image

All lanes : Anti-Caspase 9 cleaved Asp315 antibody - at 1/500 dilution

Lysates/proteins at 50 μg per lane.

This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR100-414 overnight at 4°C.

Image

IR100-414 anti-Caspase 9 cleaved Asp315 antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR100-414 anti- Caspase 9 cleaved Asp315 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Image

IR100-414 anti-Caspase 9 cleaved Asp315 antibody IHC image

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Caspase 9 cleaved Asp315 with IR100-414 at 1/200.