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IR6-17 PAX6 antibody3
Home Products Primary antibody IR6-17 PAX6 antibody
IR6-17

IR6-17 PAX6 antibody

Overview

Product Name

PAX6 antibody

Product Number

IR6-17

Gene Description

paired box 6

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse, rat

Recommended

Applications Dilutions

Western Blot 1:500

Immunofluorescence 1:200 – 1:300

Storage Buffer

100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR6-17 anti-PAX6 antibody WB image

IR6-17 anti-PAX6 antibody ICC/IF image

IR6-17 anti-PAX6 antibody IHC image

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All lanes : Anti-IL6 antibody - 1/500 dilution

Lysates/proteins at 60 μg per lane

This blot was produced using a 10% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with IR6-17 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR6-17 anti-PAX6 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded mouse testis labeling PAX6 with IR6-17 at 1/400.

1439600
Attachments:
IR6-17_PAX6.pdf