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IR324-1020 Toll-like Receptor 4, TLR 4 antibody3
Home Products Primary antibody IR324-1020 Toll-like Receptor 4, TLR 4 antibody
IR324-1020

IR324-1020 Toll-like Receptor 4, TLR 4 antibody

Overview

Product Name

Toll-like Receptor 4 antibody

Product Number

IR324-1020

Gene Description

toll-like receptor 4

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Recommended

Applications Dilutions

Western Blot 1:500 – 1:2000

Immunofluorescence 1:300 – 1:500

Immunohistochemistry (Paraffin) 1:200 – 1:300

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.51 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR324-1020 anti- Toll-like Receptor 4 antibody ICC/IF image

IR324-1020 anti- Toll-like Receptor 4 antibody IHC image

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Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR324-1020 anti- Toll-like Receptor 4 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Toll-like Receptor 4 with IR324-1020 at 1/200.

Image

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IR324-1020 anti- Toll-like Receptor 4 antibody WB image

All lanes : Anti- Toll-like Receptor 4 antibody at 1/1000 dilution

 

Lysates/proteins at 60 μg per lane

This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR324-1020 overnight at 4°C.

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