Home
1
Products
2
IR129-522 CHOP antibody3
Home Products Primary antibody IR129-522 CHOP antibody
IR129-522

IR129-522 CHOP antibody

Overview

Product Name

CHOP antibody

Product Number

IR129-522

Gene Description

DNA damage-inducible transcript 3 protein

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Recommended

Applications Dilutions

Western Blot 1:500 – 1:1000

Immunofluorescence 1:300 – 1:500

Immunohistochemistry (Paraffin) 1:100 – 1:200

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.28 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

a08a301ffd199ea43603375840d54856.jpg

IR129-522 anti-CHOP antibody WB image

All lanes : Anti-CHOP antibody - at 1/500 dilution

 

Lysates/proteins at 50 μg per lane

This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with IR129-522 overnight at 4°C.

Image

5d301b90198bb36ee7472c36a9f40cfd.jpg

IR129-522 anti-CHOP antibody WB image

All lanes : Anti-CHOP antibody - at 1/800 dilution

 

Lysates/proteins at 50 μg per lane

This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with IR129-522 overnight at 4°C.

Image

8d1f86e1a7813876c9480cfc4a4b86a0.png

IR129-522 anti-CHOP antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR129-522 anti- CHOP antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Image

d831888dab166ae6fdf2a552445ca46d.jpg

IR129-522 anti-CHOP antibody IHC image

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CHOP with IR129-522 at 1/200.

1439690