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IR135-538 PERK antibody3
Home Products Primary antibody IR135-538 PERK antibody
IR135-538

IR135-538 PERK antibody

Overview

Product Name

PERK antibody

Product Number

IR135-538

Gene Description

eukaryotic translation initiation factor 2-alpha kinase 3

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Recommended

Applications Dilutions

Western Blot 1:500

Immunofluorescence 1:300 – 1:500

Immunohistochemistry (Paraffin) 1:100 – 1:200

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.59 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

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IR135-538 anti-PERK antibody WB image

All lanes : Anti-PERK antibody - at 1/500 dilution

 

Lysates/proteins at 60 μg per lane

This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with IR135-538 overnight at 4°C.

Image

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IR135-538 anti-PERK antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR135-538 anti- PERK antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Image

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IR135-538 anti-PERK antibody IHC image

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PERK with IR135-538 at 1/200.

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Attachments:
IR135-538_PERK.pdf