IR239-895 CD138, syndecan-1 antibody
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Overview |
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Syndecan-1/ CD138 antibody |
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IR239-895 |
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syndecan 1 / SDC1 |
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Polyclonal |
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Rabbit |
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Human, Mouse |
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Western Blot 1:500 - 1:1000 Immunofluorescence 1:200 - 1:400 Immunohistochemistry (Paraffin) 1:200 - 1:300 |
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100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
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1.14 mg/ml |
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Affinity column purified |
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Store at +4°C for short term storage. Long time storage is recommended at -20°C |
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Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. |
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WB Image (Syndecan-1 / CD138 for cancer biomarker) |
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IR239-895 anti-Syndecan-1 / CD138 antibody WB image |
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All lanes : Anti- Syndecan-1 / CD138 antibody at 1/1000 dilution Lysates/proteins at 40 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR239-895 overnight at 4°C. |
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WB Image (Syndecan-1 / CD138 for MM cells biomarker) |
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IR239-895 anti-Syndecan-1 / CD138 antibody WB image |
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All lanes : Anti- Syndecan-1 / CD138 antibody at 1/500 dilution Lysates/proteins at 60 μg per lane. This blot was produced using a 5&10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR239-895 overnight at 4°C. |
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WB Image |
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IR239-895 anti-Syndecan-1 / CD138 antibody WB image |
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All lanes : Anti- Syndecan-1 / CD138 antibody at 1/1000 dilution
Lysates/proteins at 50 μg per lane This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR239-895 overnight at 4°C. |
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Image |
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IR239-895 anti- Syndecan-1 / CD138 antibody ICC/IF image |
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Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR239-895 anti- CD138 / Syndecan-1 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain. |
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IHC Image |
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IR239-895 anti- Syndecan-1 / CD138 antibody IHC image |
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Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD138 / Syndecan-1 with IR239-895 at 1/200. |
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