Overview

Product Name

Occludin antibody

Product Number

IR57-193

Gene Description

occludin

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse, rat

Recommended

Applications Dilutions

Western Blot 1:500 - 1:1000

Immunofluorescence 1:200 – 1:400

Storage Buffer

100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.56 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR57-193 anti-Occludin antibody WB image

IR57-193 anti-Occludin antibody ICC/IF image

All lanes : Anti- Occludin antibody - at 1/1000 dilution

Lysates/proteins at 50 μg per lane.

This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR57-193 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR57-193 anti-Occludin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.