Overview

Product Name

PARP1 antibody

Product Number

IR101-421

Gene Description

poly (ADP-ribose) polymerase 1

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse, rat

Recommended

Applications Dilutions

Western Blot 1:1000 – 1:1500

Immunofluorescence 1:100 – 1:300

Immunohistochemistry (Paraffin) 1:100 – 1:150

Storage Buffer

100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.59 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR101-421 anti-PARP cleaved antibody WB image

IR101-421 anti-PARP cleaved antibody ICC image

IR101-421 anti-PARP cleaved antibody

IHC image

All lanes : Anti-PARP1 antibody - 1/1000 dilution. Lysates/proteins at 40 μg per lane.

This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane was then blocked an hour before being incubated with IR101-421 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR101-421 anti- PARP cleaved antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PARP1 cleaved with IR101-421 at 1/200.