Overview

Product Name

LAMP2 / CD107b, monoclonal antibody

Product Number

IRM003

Gene Description

lysosomal-associated membrane protein 2

Clonality

Monoclonal

Host

Mouse (mouse IgG2a)

Species Reactivity

Human, Mouse

Recommended

Applications Dilutions

Western Blot 1:500 – 1:1000

Immunofluorescence 1:100 – 1:300

Immunohistochemistry (Paraffin, Frozen) 1:50 – 1:150

Storage Buffer

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Concentration

0.5 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IRM003 anti-LAMP2 / CD107b monoclonal antibody WB image

IRM003 anti-LAMP2 / CD107b monoclonal antibody IHC image

Anti-LAMP2 / CD107b monoclonal antibody at 1/500 dilution

Lysates at 50 μg per lane, This blot was produced using a 12% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with IRM003 overnight at 4°C

Immunohistochemical analysis of paraffin embedded mouse tissue labeling LAMP2 / CD107b with IRM002 at 1/100 RT 1hr.

Epitope Retrieval methods: Citrate Buffer, pH 6.0

Data from: Li-Tzung Biotechnology. Co

IRM003 anti-LAMP2 / CD107b monoclonal antibody IHC image

IRM003 anti-LAMP2 / CD107b monoclonal antibody FACS image

IRM003 anti-LAMP2 / CD107b monoclonal antibody ICC image

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling LAMP2 / CD107b with IRM003 at 1/100.

Human acute myeloid leukemia cell line KG1a was fixed, permeabilized, and stained with LAMP2 / CD107b monoclonal antibody IRM003 : 1-50

Data from :  National Chiao-Tung University

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IRM003 anti-LAMP2 / CD107b antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.