IRM003 LAMP2 / CD107b, monoclonal antibody - Lysosome Marker
Overview |
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Product Name |
LAMP2 / CD107b, monoclonal antibody |
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Product Number |
IRM003 |
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Gene Description |
lysosomal-associated membrane protein 2 |
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Clonality |
Monoclonal |
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Host |
Mouse (mouse IgG2a) |
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Species Reactivity |
Human, Mouse |
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Recommended Applications Dilutions |
Western Blot 1:500 – 1:1000 Immunofluorescence 1:100 – 1:300 Immunohistochemistry (Paraffin, Frozen) 1:50 – 1:150 |
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Storage Buffer |
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
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Concentration |
0.5 mg/ml |
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Purity |
Affinity column purified |
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Storage |
Store at +4°C for short term storage. Long time storage is recommended at -20°C |
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Notes |
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. |
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IRM003 anti-LAMP2 / CD107b monoclonal antibody WB image |
IRM003 anti-LAMP2 / CD107b monoclonal antibody IHC image |
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Anti-LAMP2 / CD107b monoclonal antibody at 1/500 dilution Lysates at 50 μg per lane, This blot was produced using a 12% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with IRM003 overnight at 4°C |
Immunohistochemical analysis of paraffin embedded mouse tissue labeling LAMP2 / CD107b with IRM002 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0 Data from: Li-Tzung Biotechnology. Co |
IRM003 anti-LAMP2 / CD107b monoclonal antibody IHC image |
IRM003 anti-LAMP2 / CD107b monoclonal antibody FACS image |
IRM003 anti-LAMP2 / CD107b monoclonal antibody ICC image |
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Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling LAMP2 / CD107b with IRM003 at 1/100. |
Human acute myeloid leukemia cell line KG1a was fixed, permeabilized, and stained with LAMP2 / CD107b monoclonal antibody IRM003 : 1-50 Data from : National Chiao-Tung University |
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IRM003 anti-LAMP2 / CD107b antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain. |