Overview

Product Name

COL1A1 / Collagen I antibody

Product Number

IR292-962

Gene Description

collagen, type I

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse, rat

Recommended

Applications Dilutions

Western Blot 1:1000 - 1:1500

Immunofluorescence 1:300 – 1:500

Immunohistochemistry (Paraffin) 1:100 – 1:300

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.51 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR292-962 anti-COL1A1 / Collagen I antibody WB image

IR292-962 anti-COL1A1 / Collagen I antibody ICC/IF image

IR292-962 anti-COL1A1 / Collagen I antibody IHC image

COL1A1 / Collagen I antibody - at 1/1000 dilution

Lysates at 40 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with IR292-962 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min. Cells were stained with IR292-962 anti-COL1A1 / Collagen I antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded mouse testis labeling COL1A1 / Collagen I with IR292-962 at 1/150.