IR292-962 COL1A1 / Collagen I Antibody
Overview |
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Product Name |
COL1A1 / Collagen I antibody |
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Product Number |
IR292-962 |
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Gene Description |
collagen, type I |
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Clonality |
Polyclonal |
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Host |
Rabbit |
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Species Reactivity |
Human, mouse, rat |
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Recommended Applications Dilutions |
Western Blot 1:1000 - 1:1500 Immunofluorescence 1:300 – 1:500 Immunohistochemistry (Paraffin) 1:100 – 1:300 |
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Storage Buffer |
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
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Concentration |
1.51 mg/ml |
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Purity |
Affinity column purified |
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Storage |
Store at +4°C for short term storage. Long time storage is recommended at -20°C |
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Notes |
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. |
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IR292-962 anti-COL1A1 / Collagen I antibody WB image |
IR292-962 anti-COL1A1 / Collagen I antibody ICC/IF image |
IR292-962 anti-COL1A1 / Collagen I antibody IHC image |
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COL1A1 / Collagen I antibody - at 1/1000 dilution Lysates at 40 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with IR292-962 overnight at 4°C. |
Immunofluorescence: cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min. Cells were stained with IR292-962 anti-COL1A1 / Collagen I antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain. |
Immunohistochemical analysis of paraffin embedded mouse testis labeling COL1A1 / Collagen I with IR292-962 at 1/150. |