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Iba1 Microglia marker ( IR13-36 Iba1 antibody ) ( IRM007 Iba1 monoclonal antibody )3
Home Products Primary antibody Iba1 Microglia marker ( IR13-36 Iba1 antibody ) ( IRM007 Iba1 monoclonal antibody )
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Iba1 Microglia marker ( IR13-36 Iba1 antibody ) ( IRM007 Iba1 monoclonal antibody )



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Overview

Product Name

Iba1/AIF1 antibody

Product Number

IR13-36

Gene Description

allograft inflammatory factor 1

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse, rat

Recommended

Applications Dilutions

Western Blot 1:500 – 1:1000

Immunofluorescence 1:100 – 1:300

Immunohistochemistry (Paraffin) 1:100 – 1:300

Immunohistochemistry (Frozen) 1:100 – 1:300

Storage Buffer

100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.7 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR13-36 anti-Iba1/AIF1 antibody IHC image

IR13-36 anti-Iba1/AIF1 antibody IHC image

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Immunohistochemical analysis of paraffin embedded Mouse / Rat brain tissue labeling Iba1 with IR13-36 at 1/200.

Epitope Retrieval methods: Citrate Buffer, pH 6.0

Data from: Li-Tzung Biotechnology. Co

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Iba1 with IR13-36 at 1/200.

Epitope Retrieval methods: Citrate Buffer, pH 6.0


IR13-36 anti-Iba1/AIF1 antibody WB image

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IR13-36 anti-Iba1/AIF1 antibody IHC-Fr image

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Iba1/AIF1 antibody - at 1/500 dilution

Lysates at 45 μg per lane, This blot was produced using a 15% SDS-PAGE. PVDF membrane was then blocked with 3%BSA for an hour before being incubated with IR13-36 overnight at 4°C.

Frozen sectioned for immunohistochemical analysis.

Sample: Frozen sectioned Rat brain.

Green: Iba1 antibody (IR13-36)

Red: TUJ1 antibody

Blue: DAPI

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IR13-36 anti-Iba1/AIF1 antibody IHC-Fr image

Customer feedback Image

IR13-36 anti-Iba1/AIF1 antibody IHC-Fr image

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Immunohistochemical of frozen sections.

Sample : mouse cerebellum.

Green : Iba1 antibody (IR13-36) : 1-200

Anti-rabbit 488 : 1-500.

DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical of frozen sections.

Sample : mouse hippocampus.

Green : Iba1 antibody (IR13-36) : 1-200

Anti-rabbit 488 : 1-500.

DAPI (blue) was used as the nuclear counter stain.



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