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EpCAM in human circulating tumour cells ( IR218-3 EpCAM antibody )3
Home Products Primary antibody EpCAM in human circulating tumour cells ( IR218-3 EpCAM antibody )
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EpCAM in human circulating tumour cells ( IR218-3 EpCAM antibody )

Catalog

Component

Clonality

Reactivity

App

IR218-3

EpCAM antibody

Extracellular domain

Rab-pAb

H, M

WB, IF, IHC, FC, CTC

IR218-1

EpCAM antibody

Intracellular domain

Rab-pAb

H, M, R

WB, IF, IHC, CTC

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Catalog Number: IR218-3

Anti-EpCAM antibody (Extracellular domain)

Package size: 25, 100 μl

Store at: -20°C

MW (kDa): 34-40

Overview

Product Name

EpCAM antibody (Extracellular domain)

Product Number

IR218-3

Gene Description

epithelial cell adhesion molecule

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse

Recommended

Applications Dilutions

Western Blot 1:1000 – 1:2000

Immunofluorescence 1:200 – 1:400

Immunohistochemistry (Paraffin) 1:100 – 1:150

Flow Cytometry 1:50 – 1:200

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.54 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR218-3 anti-EpCAM antibody WB image

IR218-3 anti- EpCAM antibody IHC image

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Anti-EpCAM antibody - at 1/1000 dilution

Lysates at 40 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR218-3 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with IR218-3 at 1/200 RT 1hr.

IR218-3 anti- EpCAM antibody FACS image

IR218-3 anti- EpCAM antibody ICC/IF image

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ProductEpCAM antibody

CatalogIR218-3

Cell : MCF-7

Data from :  National Chiao-Tung University

Cell : MCF-7   (DAPI : 1-300, Phalloidin : 1-300)

EpCAM, (IR218-3) : 1-200

Anti-rabbit 594 : 1-500

NikonEpi-fluorescence (A-ken) 60x

Data from : National Yang-Ming University

 

IR218-3 anti-EpCAM antibody WB image

IR218-3 anti- EpCAM antibody IHC image

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Anti-EpCAM antibody - at 1/1000 dilution

Lysates at 40 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR218-3 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling EpCAM with IR218-3 at 1/200 RT 1hr.

Epitope Retrieval methods: Citrate Buffer, pH 6.0

Data from: Li-Tzung Biotechnology. Co

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