iReal Biotechnology, Inc.

Products

Custom Antibodies

Polyclonal antibodies are a heterogeneous mix of immunoglobulins derived from various B cells that individually target distinct epitopes on an immunogen. All antibodies are made to standards outlined in quality management systems ISO 13485:2016, resulting in exceptional manufacturing and quality control expertise that translates into reliable antibody reagents. Let . iReal Biotechnology help you develop the ideal polyclonal antibodies to accelerate your research progress.

Custom Polyclonal Antibody Production

Using a defined immunization, boost and bleed schedule, and purification methods, we develop highly specific polyclonal antibodies for your application. We offer polyclonal development rabbits and can convert to recombinant monoclonal antibodies if the need arises to secure long term supply.

Our custom polyclonal antibody process includes:

Immunize, boost, and test bleed

Production bleeds

Serum pooling

Purification

QC Testing

Key Design and Production Features

Precise Antigen Design

Professional epitope design to optimize immunogen synthesis, solubility and antigenicity properties.

Production of immunogens

Efficient production of high-quality peptide or protein immunogens.

High efficiency immunization

Enhanced cocktail immunization techniques.

Specific purification

Antigen-affinity chromatography purification of antibodies.

Good Practice For Antibody Manufacturing

All antibodies are made to standards outlined in quality management systems ISO 13485:2016

Our Custom Polyclonal Antibody Production Process
Each project is performed in distinct phases—and you decide if and when to initiate each phase.

Phase 1: Antigen Design and Preparation

Precise epitope Design
Protein, recombinant or purified
Synthetic peptide Modified peptide

Phase 2: Polyclonal Antibody Development

During polyclonal antibody development, we immunize based on the schedule you request and can recommend an adjuvant to achieve your project goals. We provide a pre-bleed serum for screening in your labs and can also use our experience and monitoring of the antibody-mediated immune response to make recommendations prior to the collection of production bleeds.

Procdeure	Description
Control Serum Collection	Pre-immune bleed (3 mL per rabbit)
Immunization	Protein antigen: 3-4 times Peptide antigen: 5-6 times
Serum Collection	Obtain anti-serum (40 mL per rabbit)
Anti-serum verify	Verified by Dot blot or specific WB
Antibody purification	Antibody purified by antigen affinity
Antibody verify	Verified by WB or other application

CustomFlow Chart for Polyclonal Antibody Production

Products	Description
Serum	Pre-immune bleed (3 mL per rabbit)
Antibody	3-5 mg antibody (antigen purified)
CoA	Report of antibody validation Report of antigen by LC-MS
Antigen	Design sequence