Posters

Selectins : Trafficking of immune system cells ( IR306-909 L-selectin IR307-912 P-selectin IR308-917 E-selectin )

Selectins are required for the trafficking of innate immune system cells, T lymphocytes and platelets. The absence of selectins or their ligands has severe health consequences, with recurrent bacterial infections and persistent disease occurring. During inflammation, selectins enable the initial attachment of leukocytes from the bloodstream, which causes their downstream movement along the endothelium via adhesive interactions referred to as leukocyte rolling. Three type-I-cell surface glycoproteins make up the selectin family, namely L-selectin, P-selectin and E-selectin. L-selectin is the smallest of the selectins and is expressed on all granulocytes and monocytes and the majority of lymphocytes. P-selectin is the largest selectin and is found on the surface of activated endothelial cells and platelets. The expression of E-selectin does not occur under baseline conditions, aside from in skin microvessels, but its expression on activated endothelium is induced by inflammatory cytokines.

Catalog	Component	Clonality	Reactivity	App
IR306-909	L-selectin / CD62L	Rab-pAb	H, M, R	WB, IF, IHC
IR307-912	P-selectin / CD62P	Rab-pAb	H, M, R	WB, IF, IHC
IR308-917	E-selectin / CD62E	Rab-pAb	H, M, R	WB, IF, IHC

P-selectin / CD62P antibody IR307-912

E-selectin / CD62E antibody IR308-917

Overview
Product Name	L-selectin / CD62L antibody
Product Number	IR306-909
Gene Description	selectin, lymphocyte
Clonality	Polyclonal
Host	Rabbit
Species Reactivity	Human, mouse, rat
Recommended Applications Dilutions	Western Blot 1:500-1:1000 Immunofluorescence 1:200 – 1:500
Storage Buffer	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Concentration	0.84 mg/ml
Purity	Affinity column purified
Storage	Store at +4°C for short term storage. Long time storage is recommended at -20°C
Notes	Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR306-909 anti L-selectin / CD62L antibody WB image	IR306-909 anti L-selectin / CD62L antibody IHC image

All lanes : Anti L-selectin / CD62L antibody at 1/500 dilution. Lysates at 50 μg per lane, This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR306-909 overnight at 4°C.	Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling L-selectin / CD62L with IR306-909 at 1/100.

Overview
Product Name	P-selectin / CD62P antibody
Product Number	IR307-912
Gene Description	selectin P (granule membrane protein 140kDa, antigen CD62)
Clonality	Polyclonal
Host	Rabbit
Species Reactivity	Human, mouse, rat
Recommended Applications Dilutions	Western Blot 1:500-1:1000 Immunofluorescence 1:200 – 1:500
Storage Buffer	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Concentration	1.51 mg/ml
Purity	Affinity column purified
Storage	Store at +4°C for short term storage. Long time storage is recommended at -20°C
Notes	Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
IR307-912 anti P-selectin / CD62P antibody WB image		IR307-912 anti P-selectin / CD62P antibody IHC image

All lanes : Anti P-selectin / CD62P antibody at 1/500 dilution Lysates at 50 μg per lane, This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR307-912 overnight at 4°C.		Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling P-selectin / CD62P with IR307-912 a

Overview
Product Name	E-selectin / CD62E antibody
Product Number	IR308-917
Gene Description	selectin E
Clonality	Polyclonal
Host	Rabbit
Species Reactivity	Human, mouse, rat
Recommended Applications Dilutions	Western Blot 1:500-1:1000 Immunofluorescence 1:200 – 1:500
Storage Buffer	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Concentration	1.33 mg/ml
Purity	Affinity column purified
Storage	Store at +4°C for short term storage. Long time storage is recommended at -20°C
Notes	Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
IR308-917 anti E-selectin / CD62E antibody WB image		IR308-917 anti E-selectin / CD62E antibody IHC image

All lanes : Anti E-selectin / CD62E antibody at 1/500 dilution Lysates at 50 μg per lane, This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR308-917 overnight at 4°C.		Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling E-selectin / CD62E with IR308-917 at 1/100.