Products
Products
Custom Antibodies
Polyclonal antibodies are a heterogeneous mix of immunoglobulins derived from various B cells that individually target distinct epitopes on an immunogen. All antibodies are made to standards outlined in quality management systems ISO 13485:2016, resulting in exceptional manufacturing and quality control expertise that translates into reliable antibody reagents. Let . iReal Biotechnology help you develop the ideal polyclonal antibodies to accelerate your research progress.
Custom
Polyclonal Antibody Production
Immunize, boost, and test bleed
Production bleeds
Serum pooling
Purification
QC Testing
Key
Design and Production Features
Using a defined immunization, boost and bleed schedule, and
purification methods, we develop highly specific polyclonal antibodies for
your application. We offer polyclonal development rabbits and can convert
to recombinant monoclonal antibodies if the need arises to secure long
term supply.
Our custom polyclonal antibody process includes:
Our custom polyclonal antibody process includes:
1.Precise
Antigen
Design
Professional epitope
design to optimize immunogen synthesis, solubility and antigenicity
properties.
2.
Production
of immunogens
Efficient production of high-quality peptide or protein immunogens.
Efficient production of high-quality peptide or protein immunogens.
3.
High
efficiency immunization
Enhanced cocktail immunization techniques.
Enhanced cocktail immunization techniques.
4.
Specific
purification
Antigen-affinity chromatography purification of antibodies.
Antigen-affinity chromatography purification of antibodies.
5.
Good Practice For Antibody Manufacturing
All antibodies are made to standards outlined in quality management systems ISO 13485:2016
All antibodies are made to standards outlined in quality management systems ISO 13485:2016
Our Custom Polyclonal Antibody Production Process
Each project is performed in distinct phases—and you decide if and when to initiate each phase.
Each project is performed in distinct phases—and you decide if and when to initiate each phase.
Phase 1: Antigen Design and Preparation
- Precise epitope Design
- Protein, recombinant or purified
- Synthetic peptide Modified peptide
Phase 2: Polyclonal Antibody Development
During polyclonal antibody development, we immunize based on the schedule you request and can recommend an adjuvant to achieve your project goals. We provide a pre-bleed serum for screening in your labs and can also use our experience and monitoring of the antibody-mediated immune response to make recommendations prior to the collection of production bleeds.
| Procdeure | Description |
| Control Serum Collection | Pre-immune bleed (3 mL per rabbit) |
| Immunization |
Protein antigen: 3-4 times Peptide antigen: 5-6 times |
| Serum Collection | Obtain anti-serum (40 mL per rabbit) |
| Anti-serum verify | Verified by Dot blot or specific WB |
| Antibody purification | Antibody purified by antigen affinity |
| Antibody verify | Verified by WB or other application |
CustomFlow Chart for Polyclonal Antibody Production
| Products |
Description |
| Serum |
Pre-immune bleed (3 mL per rabbit) |
| Antibody |
3-5 mg antibody (antigen purified) |
| CoA |
Report of antibody validation Report of antigen by LC-MS |
| Antigen |
Design sequence |