CD142 / coagulation factor III / tissue factor / F3 : Hemostasis in health and disease ( IR295-1, IR295-2 Tissue Factor / CD142 antibody ) )
CD142, coagulation factor III, tissue factor, F3. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. Platelets and monocytes have been shown to express this coagulation factor under pro-coagulatory and proinflammatory stimuli, and a major role in HIV-associated coagulopathy has been described. Platelet-dependent monocyte expression of coagulation factor III has been described to be associated with Coronavirus Disease 2019 (COVID-19) severity and mortality.
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Catalog |
Component |
Clonality |
Reactivity |
App |
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Tissue Factor / CD142 extracellular |
Rab-pAb |
Human |
WB, IF, IHC |
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Tissue Factor / CD142 |
Rab-pAb |
Human |
WB, IF, IHC |
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Overview |
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Product Name |
Tissue Factor / CD142 extracellular antibody |
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Product Number |
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Clonality |
Polyclonal |
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Host |
Rabbit |
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Species Reactivity |
Human |
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Recommended Applications Dilutions |
Western Blot 1:1000 - 1:2000 Immunofluorescence 1:300 – 1:600 Immunohistochemistry (Paraffin) 1:100 – 1:300 |
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Storage Buffer |
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 |
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Concentration |
1.5 mg/ml |
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Purity |
Affinity column purified |
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Storage |
Store at +4°C for short term storage. Long time storage is recommended at -20°C |
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Notes |
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. |
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IR295-1 anti-Tissue Factor / CD142 antibody WB image |
IR295-1 anti-Tissue Factor / CD142 antibody IHC image |
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Anti- Tissue Factor / CD142 antibody - at 1/1000 dilution. Lysates at 30 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR295-1 overnight at 4°C. |
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Tissue Factor / CD142 with IR295-1 at 1/100. |
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