Product Includes
Application Dilution
Immunofluorescence 1:200–1:400
Immunohistochemistry (Paraffin) 1:100-1:150
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. [provided by RefSeq, Aug 2017]
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Reviews
IR127-515 anti-IRE1 alpha antibody WB image
Anti-IRE1 alpha antibody - at 1/500 dilution, Lysates at 50 μg per lane.
This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with IR127-515 overnight at 4°C.
IR127-515 anti-IRE1 alpha antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse testis labeling IRE1 alpha with IR127-515 at 1/100.
IR127-515 anti-IRE1 alpha antibody ICC/IF image
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with IR127-515 anti- IRE1 alpha antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.