Product Includes
Application Dilution
Immunofluorescence 1:200–1:500
Immunohistochemistry (Paraffin) 1:100-1:150
Storage
100mM Tris Glycine, 1%BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
| Title | |
|---|---|
| No data. | |
Reviews
IR133-535 anti-eIF2 alpha antibody WB image
Anti-eIF2 alpha antibody - at 1/500 dilution, Lysates at 50 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked with 3%BSA an hour before being incubated with IR133-535 overnight at 4°C.
IR133-535 anti-eIF2 alpha antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR133-535 anti- eIF2 alpha antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR133-535 anti-eIF2 alpha antibody IHC image
Immunohistochemical analysis of paraffin embedded breast tissue labeling eIF2 alpha with IR133-535 at 1/100.