Product Includes
Application Dilution
Immunofluorescence 1:100-1:200
Immunohistochemistry (Paraffin) 1:100-1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015]
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Reviews
Customer feedback Image
Application : FFPE immunohistochemistry
Condition / Dilution： 1-300
Epitope Retrieval methods: Citrate Buffer, pH 6.0
Sample : mouse liver
Data from : Li-Tzung Biotechnology. Co
Customer feedback Image
Application : Western blot
Condition / Dilution： 1-1000
Sample : HCT-116 drug treatment
Data from : Taipei Medical University
IR136-543 anti-NRF2 antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse lung labeling NRF2 with IR136-543 at 1/200 RT 1hr.Epitope Retrieval methods: Citrate Buffer, pH 6.0
IR136-543 anti-NRF2 antibody WB image
Anti-NRF2 antibody at 1/800 dilution.
Lysates at 30 μg per lane. This blot was produced using a 5% SDS-PAGE. NC membrane was then blocked for an hour before being incubated with IR136-543 overnight at 4°C.
IR136-543 anti-NRF2 antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse liver labeling NRF2 with IR136-543 at 1/200 RT 1hr.Epitope Retrieval methods: Citrate Buffer, pH 6.0
IR136-543 anti-NRF2 antibody_Mouse Kidney IHC image
Immunohistochemical analysis of paraffin embedded mouse Kidney tissue labeling NRF2 with IR136-543 at 1/100 RT 1hr.
Epitope Retrieval methods: Citrate Buffer, pH 6.0
IR136-543 anti-NRF2 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR136-543 anti-NRF2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR136-543 anti-NRF2 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR136-543 anti-NRF2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR136-543 anti-NRF2 antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse tissue labeling NRF2 with IR136-543 at 1/100 RT 1hr.
Epitope Retrieval methods: Citrate Buffer, pH 6.0
IR136-543 anti-NRF2 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling NRF2 with IR136-543 at 1/150.
IR136-543 anti-NRF2 antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse tissue labeling NRF2 with IR136-543 at 1/100 RT 1hr.
Epitope Retrieval methods: Citrate Buffer, pH 6.0
IR136-543 anti-NRF2 antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse tissue labeling NRF2 with IR136-543 at 1/100 RT 1hr.
Epitope Retrieval methods: Citrate Buffer, pH 6.0
IR136-543 anti-NRF2 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling NRF2 with IR136-543 at 1/150.
IR136-543 anti-NRF2 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling NRF2 with IR136-543 at 1/150.
IR136-543 anti-NRF2 antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse tissue labeling NRF2 with IR136-543 at 1/100 RT 1hr.
Epitope Retrieval methods: Citrate Buffer, pH 6.0
IR136-543 anti-NRF2 antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse tissue labeling NRF2 with IR136-543 at 1/100 RT 1hr.
Epitope Retrieval methods: Citrate Buffer, pH 6.0