Exosome
CD31
Antibodies Panel
IR171-666 anti-AKT (pan) antibody WB image IR171-666 anti-AKT (pan) antibody WB image IR171-666 anti-AKT (pan) antibody ICC/IF image IR171-666 anti-AKT (pan) antibody ICC/IF image
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Catalog Number:IR171-666

AKT (pan) antibody

Application
  • WB
  • IF
Reactivity Hu、Ms、Rat

REACTIVITYHu、Ms、Rat
SENSITIVITYEndogenous
MW60
SOURCERabbit-IgG

Product Includes

-

Application Dilution

Western Blot 1:1000-1:2000
Immunofluorescence 1:200-1:500

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C
100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Specificity / Sensitivity

Endogenous

Source / Immunogen

Synthetic peptide / encompassing a sequence within the C-terminus region.

This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2020]

Title
1

Huang, Xiao-Fan et al. “Juniperus indica Bertol. extract synergized with cisplatin against melanoma cells via the suppression of AKT/mTOR and MAPK signaling and induction of cell apoptosis.” International journal of medical sciences vol. 18,1 157-168. 1 Jan. 2021

Application
  • WB
Reactivity Human
2

Huang, Xiao-Fan et al. “Extract Derived from Cedrus atlantica Acts as an Antitumor Agent on Hepatocellular Carcinoma Growth In Vitro and In Vivo.” Molecules (Basel, Switzerland) vol. 25,20 4608. 10 Oct. 2020

Application
  • WB
Reactivity Human
3

Jadhao, Mahendra et al. “The Long-Term DEHP Exposure Confers Multidrug Resistance of Triple-Negative Breast Cancer Cells through ABC Transporters and Intracellular ROS.” Antioxidants (Basel, Switzerland) vol. 10,6 949. 11 Jun. 2021

Application
  • WB
Reactivity Human
4

Liang, Chi-Yen et al. “Patchouli alcohol induces G0 /G1 cell cycle arrest and apoptosis in vincristine-resistant non-small cell lung cancer through ROS-mediated DNA damage.” Thoracic cancer, 10.1111/1759-7714.14982. 30 May. 2023

Application
  • WB
  • ICC/IF
Reactivity Human
5

Chang, Kai-Fu et al. “Downregulation of VEGFR2 signaling by cedrol abrogates VEGF‑driven angiogenesis and proliferation of glioblastoma cells through AKT/P70S6K and MAPK/ERK1/2 pathways.” Oncology letters vol. 26,2 342. 22 Jun. 2023

Application
  • WB
Reactivity Human
6

Hua, Wei-Jyun et al. “Ganoderma microsporum immunomodulatory protein combined with KRASG12C inhibitor impedes intracellular AKT/ERK network to suppress lung cancer cells with KRAS mutation.” International journal of biological macromolecules vol. 259,Pt 2 (2024)

Application
  • WB
Reactivity Human
7

Peng, Min et al. “The IMPDH cytoophidium couples metabolism and fetal development in mice.” Cellular and molecular life sciences : CMLS vol. 81,1 210. 8 May. 2024

Application
  • WB
Reactivity Mouse

Reviews

Customer Feedback
2022-10-06 09:17:32
0
0
Customer Feedback
2022-10-06 09:17:32
0
0

Customer feedback Image
Application : Western blot
Condition / Dilution:1-1000, 5%BSA/TBST
Sample : HCC827
Data from : National Yang Ming Chiao Tung University

Customer Feedback
2022-10-06 09:16:06
0
0
Customer Feedback
2022-10-06 09:16:06
0
0

Customer feedback Image
Application : Western blot
Condition / Dilution: 1-1000, 5%milk
Sample : H1975
Data from : National Yang Ming Chiao Tung University

Customer Feedback
2022-10-06 09:15:08
0
0
Customer Feedback
2022-10-06 09:15:08
0
0

Customer feedback Image
Application : Western blot
Condition / Dilution: 1-1000, 5%milk
Sample : MCF-7 / MDA-MB-231 drug treatment
Data from : Kaohsiung Medical University

Customer Feedback
2022-10-06 09:13:50
0
0
Customer Feedback
2022-10-06 09:13:50
0
0

Customer feedback Image
Application : Western blot
Condition / Dilution: 1-1000
Sample : A172
Data from : Taipei Medical University

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