Product Includes
Application Dilution
Immunofluorescence 1:100-1:400
Immunohistochemistry (Paraffin) 1: 100-1:200
Storage
100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]
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Customer feedback Image
Application : ICC/IF
Condition / Dilution：1-200
Sample : Cardiomyocytes
Data from : Taipei Medical University
IR91-384 anti-SQSTM1 / p62 antibody
Anti-SQSTM1 / p62 antibody - at 1/1000 dilution. Lysates at 35 μg per lane.
This blot was produced using a 12% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with IR91-384 overnight at 4°C.
IR91-384 anti-SQSTM1 / p62 antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse kidney labeling SQS/TM1 / P62 with IR91-384 at 1/200 RT 1hr.Epitope Retrieval methods: Citrate Buffer, pH 6.0
IR91-384 anti-SQSTM1 / p62 antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse spleen labeling SQS/TM1 / P62 with IR91-384 at 1/200 RT 1hr.Epitope Retrieval methods: Citrate Buffer, pH 6.0
IR91-384 anti-SQSTM1 / p62 antibody ICC image
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR91-384 anti- SQSTM1 / p62 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR91-384 anti-SQSTM1 / p62 antibody_Colorectal carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human Colorectal cancer tissue labeling SQSTM1 / p62 with IR91-384 at 1/200.
IR91-384 anti-SQSTM1 / p62 antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse tissue labeling SQS-TM1 / p62 with IR91-384 at 1/100 RT 1hr.
Epitope Retrieval methods: Citrate Buffer, pH 6.0
IR91-384 anti-SQSTM1 / p62 antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse tissue labeling SQS-TM1 / p62 with IR91-384 at 1/100 RT 1hr.
Epitope Retrieval methods: Citrate Buffer, pH 6.0
IR91-384 anti-SQSTM1 / p62 antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse tissue labeling SQS-TM1 / p62 with IR91-384 at 1/100 RT 1hr.
Epitope Retrieval methods: Citrate Buffer, pH 6.0
IR91-384 anti-SQSTM1 / p62 antibody_HT-29 ICC/ IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR91-384 anti- SQSTM1 / p62 antibody (red) at 1:100 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR91-384 anti-SQSTM1 / p62 antibody_MDA-MB-231 ICC/ IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR91-384 anti- SQSTM1 / p62 antibody (red) at 1:100 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR91-384 anti-SQSTM1 / p62 antibody_HUVEC ICC/ IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR91-384 anti- SQSTM1 / p62 antibody (red) at 1:100 and 4°C. DAPI (blue) was used as the nuclear counter stain.