Product Includes
Application Dilution
Immunohistochemistry (Paraffin) 1:100 – 1:200
Flow Cytometry 1:50 – 1:100
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
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Reviews
WB Customer feedback Image
Dilution : 1-1000
Sample : Human TNBC plasma_Triple-negative breast cancer
WB Customer feedback Image
Dilution : 1-1000
Sample : Human Breast Cancer Buffy coat
IR302-986 anti-CD81 antibody WB image
Anti-CD81 antibody at 1/500 dilution
Lysates/proteins at 65 μg per lane
This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR302-986 overnight at 4°C.
IR302-986 anti-CD81 antibody WB image
Anti-CD81 antibody at 1/500 dilution
Lysates at 40 μg per lane
This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR302-986 overnight at 4°C.
IR302-986 anti-CD81 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD81 with IR302-986 at 1/100.
Customer Feedback IR302-986 anti-CD81 antibody WB image
Anti-CD81 antibody at 1/500 dilution
Lysates at 50 μg per lane
This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR302-986 overnight at 4°C.
IR302-986 anti-CD81 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR302-986 anti- CD81 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR302-986 anti-CD81 antibody WB image
Anti-CD81 antibody at 1/500 dilution
Lysates at 50 μg per lane
This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR302-986 overnight at 4°C.