Product Includes
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Application Dilution
Western Blot 1:3000-1:7000
Immunofluorescence 1:500-1:1000
Immunofluorescence 1:500-1:1000
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Endogenous
Source / Immunogen
Synthetic peptide (YPYDVPDYA)
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#IRT003
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DYKDDDDK-tag antibody
#IRT002
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His tag antibody
#IRT008
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GST-tag [6D] monoclonal antibody
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Reviews
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IRT001 anti HA-tag antibody WB image
All lanes : Anti HA-tag antibody at 1/5000 dilution
Lysates/proteins at 30 μg per lane
This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IRT001 overnight at 4°C.