Product Includes
Application Dilution
Immunofluorescence 1:100 – 1:300
Immunohistochemistry (Paraffin) 1:50 – 1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]
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Reviews
IR81-279 anti-VEGF Receptor 2 antibody WB image
VEGF Receptor 2 antibody - at 1/500 dilution
Lysates at 50 μg per lane, This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with IR81-279 overnight at 4°C.
IR81-279 anti-VEGF Receptor 2 antibody WB image
VEGF Receptor 2 antibody - at 1/500 dilution
Lysates at 50 μg per lane, This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with IR81-279 overnight at 4°C.
IR81-279 anti-VEGF Receptor 2 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling VEGF Receptor 2 with IR81-279 at 1/200.
IR81-279 anti-VEGF Receptor 2 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR81-279 anti- VEGF Receptor 2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR81-279 anti-VEGF Receptor 2 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling VEGF Receptor 2 with IR81-279 at 1/100.