Catalog Number：IRBS4022
Anti-Mouse IgG (H+L) (GOAT) Antibody Peroxidase Conjugated
Application
Reactivity Mouse IgG
Product Includes
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Application Dilution
Western Blot 1:5,000-1:10,000
ELISA 1:20,000–1:40,000
Immunohistochemistry 1:500–1:2,000
Other Assays User Optimized
ELISA 1:20,000–1:40,000
Immunohistochemistry 1:500–1:2,000
Other Assays User Optimized
Storage
Long time storage is recommended at -20°C
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA), 50% Glycerol, 0.025% ProClin 300 was added as a preservative, Do NOT add Sodium Azide!
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA), 50% Glycerol, 0.025% ProClin 300 was added as a preservative, Do NOT add Sodium Azide!
Specificity / Sensitivity
Endogenous
Source / Immunogen
Designed for use with mouse polyclonal and monoclonal antibodies, this affinity purified goat anti-mouse IgG (heavy and light chain) antibody is conjugated to horseradish peroxidase(HRP) for chemilumi
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#IRBS4012
Rabbit IgG
Anti-Rabbit IgG (H+L) (GOAT) Antibody Peroxidase Conjugated
#IRBS4032
Rat IgG
Anti-Rat IgG (H+L) (GOAT) Antibody Peroxidase Conjugated
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Reviews
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Anti-Mouse IgG (H+L) (GOAT) Antibody Peroxidase Conjugated
Lysates at 50 ng : Mouse IgG. (Denature form)
Primary antibody: none.
Secondary antibody: Peroxidase Conjugated Goat Anti- Mouse IgG Antibody (Catalog. IRBS4022) at 1:7,000 for 60 min at RT.
Observed size: 50 kDa, 25 kDa for Mouse IgG.
Anti-Mouse IgG (H+L) (GOAT) Antibody Peroxidase Conjugated
Lysates at 30 μg per lane. The membrane was then blocked for an hour before being incubated with IRM002 PCNA mouse monoclonal antibody overnight at 4°C. Secondary antibody: Peroxidase Conjugated Goat Anti-Mouse IgG Antibody (Catalog. IRBS4022) at 1:7,000 for 60 min at RT.