Product Includes
Application Dilution
Immunofluorescence 1:200–1:400
Immunohistochemistry (Paraffin) 1: 100-1:300
Immunohistochemistry (Frozen) 1:100-1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]
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Atalog Number : IR82-282
Dilution : 1:400
Reactivity : immunofluorescent
Sample : rat penis
Red : eNOS / NOS3 antibody
Anti-rabbit 594 : 1-400
Fixed: 4% paraformaldehyde at RT for 50 min
IR82-282 anti-eNOS / NOS3 antibody WB image
Anti- eNOS / NOS3 antibody - at 1/1000 dilution
Lysates/proteins at 40 μg per lane
This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with IR82-282 overnight at 4°C.
IR82-282 anti-eNOS / NOS3 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling eNOS with IR82-282 at 1/200.
IR82-282 anti-eNOS / NOS3 antibody IHC image Customer feedback Image
Frozen sectioned for immunohistochemical analysis.
Sample: Frozen section.
Red: eNOS / NOS3 antibody.
Catalog: IR82-282 at 1-100
IR82-282 anti-eNOS / NOS3 antibody WB image
All lanes : Anti- eNOS / NOS3 antibody - at 1/1000 dilution
Lysates/proteins at 40 μg per lane
This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with IR82-282 overnight at 4°C.
IR82-282 anti-eNOS / NOS3 antibody WB image
All lanes : Anti- eNOS / NOS3 antibody - at 1/1000 dilution
Lysates/proteins at 40 μg per lane
This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with IR82-282 overnight at 4°C.
IR82-282 anti-eNOS / NOS3 antibody_HepG2 ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT,
permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30
min at room temperature. Cells were stained with IR82-282 anti- eNOS/ NOS3
antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.