Product Includes
Application Dilution
Immunofluorescence 1:100-1:200
Immunohistochemistry (Paraffin) 1:100-1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]
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Application
Reactivity Human
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Reactivity Mouse
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Application : Western blot
Condition / Dilution： 1-500
Sample : HCT-116 drug treatment
Data from : Taipei Medical University
IR113-466 anti-HIF1 alpha antibody WB image
Anti-HIF1 alpha antibody at 1/500 dilution. Lysates at 30 μg per lane
This blot was produced using a 7.5% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with IR113-466 overnight at 4°C.
IR113-466 anti-HIF1 alpha antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with IR113-466 anti- HIF1 alpha antibody (red) at 1:200 and 4°C.
IR113-466 anti-HIF1 alpha antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling HIF1 alpha with IR113-466 at 1/200.