Product Includes
Application Dilution
Immunofluorescence 1:200–1:500
Immunohistochemistry (Paraffin) 1: 100-1:200
Storage
100mM Tris Glycine, 1%BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]
Reviews
Customer feedback Image
Application : Western blot
Condition / Dilution：1-1000
Sample : HCT-116 drug treatment
Data from : Taipei Medical University
Customer feedback Image
Application : Western blot
Condition / Dilution：1-1000, 5%milk
Sample : MCF-7 / MDA-MB-231 drug treatment
Data from : Kaohsiung Medical University
Customer feedback Image
Application : Western blot
Condition / Dilution：1-1000, 5%milk
Sample : Human cancer cell
Data from : National Yang Ming Chiao Tung University
IR208-752 anti-CD44 antibody WB image
Anti-CD44 antibody - at 1/1000 dilution
Lysates at 40 μg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR208-752 overnight at 4°C.
IR208-752 anti-CD44 antibody WB image
Anti-CD44 antibody - at 1/1000 dilution
Lysates at 40 μg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR208-752 overnight at 4°C. Vimentin antibody (IR45-137).
IR208-752 anti-CD44 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD44 with IR208-752 at 1/150.
IR208-752 anti-CD44 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD44 with IR208-752 at 1/150.
IR208-752 anti-CD44 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD44 with IR208-752 at 1/150.
IR208-752 anti-CD44 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD44 with IR208-752 at 1/150.
IR208-752 anti-CD44 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR208-752 anti- CD44 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR208-752 anti-CD44 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR208-752 anti- CD44 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.