Product Includes
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Application Dilution
Western Blot 1:1000 - 1:3000
Immunofluorescence 1:300 – 1:600
Immunohistochemistry (Paraffin) 1:100 – 1:150
Immunofluorescence 1:300 – 1:600
Immunohistochemistry (Paraffin) 1:100 – 1:150
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Endogenous
Source / Immunogen
Synthetic peptide / encompassing a sequence within the center region
The protein encoded by this gene belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. This family member is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2010]
IR291-959 anti IRF-4 / MUM1 antibody WB image
Anti IRF-4 / MUM1 antibody - at 1/2000 dilution
Lysates at 30 μg per lane. This blot was produced using a 7.5% SDS-PAGE.
Nitrocellulose membrane was then blocked for an hour before being incubated with IR291-959 overnight at 4°C.
IR291-959 anti IRF-4 / MUM1 antibody_Human tonsil cancer tissue IHC image
Immunohistochemical analysis of paraffin embedded Human tonsil cancer tissue labeling IRF-4 / MUM1 with IR291-959 at 1/150.