Product Includes
Application Dilution
Immunofluorescence 1:200–1:300
Immunohistochemistry (Paraffin) 1: 100-1:150
Storage
100mM Tris Glycine, 40% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]
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Reviews
IR289-955 anti-CTGF antibody WB image
Anti-CTGF antibody at 1/700 dilution
Lysates at 50 μg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR289-955 overnight at 4°C.
IR289-955 anti-CTGF antibody WB image
Anti-CTGF antibody at 1/700 dilution
Lysates at 50 μg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR289-955 overnight at 4°C.
IR289-955 anti-CTGF antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR289-955 anti-CTGF antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR289-955 anti-CTGF antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CTGF with IR289-955 at 1/100.