![IRM319 Glycophorin A / CD235a antibody [M520] WB Image](/Upload/Products/1679034729_c83446ef5c8bd6df3259.jpg "IRM319 Glycophorin A / CD235a antibody [M520] WB Image")
Product Includes
Application Dilution
Immunofluorescence 1:200 – 1:500
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Specificity / Sensitivity
Source / Immunogen
Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]
IRM319 Glycophorin A / CD235a antibody [M520] WB Image
This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IRM319 overnight at 4°C.
Lysates at 30 μg per lane.
Anti-Glycophorin A / CD235a antibody [M520]at 1/1000 dilution