Product Includes
Application Dilution
Immunofluorescence 1:300 – 1:500
Immunohistochemistry (Frozen) 1:100 – 1:300
Immunohistochemistry (Paraffin) 1:100 – 1:300
Flow Cytometry 1:50 – 1:150
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Specificity / Sensitivity
Source / Immunogen
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
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Reviews
IR75-253 anti-mouse CD31 antibody WB image
Lysates at 30 μg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane was then blocked for an hour before being incubated with IR75-253 overnight at 4°C.
Anti-mouse CD31 antibody at 1/1000 dilution
IR75-253 anti-mouse CD31 antibody IHC-Fr image
Immunohistochemical of frozen sections.
Sample : mouse tissue.
Mouse CD31 antibody : 1-200
Blue : DAPI was used as the nuclear counter stain.
IR75-253 anti-mouse CD31 antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse and human placenta tissue labeling mouse CD31 antibody with IR75-253 at 1/200.
IR75-253 anti-mouse CD31 antibody IHC-P image
Immunohistochemical analysis of paraffin embedded mouse placenta tissue labeling mouse CD31 antibody with IR75-253 at 1/200.
IR75-253 anti-mouse CD31 antibody IHC-P image
Immunohistochemical analysis of paraffin embedded mouse placenta tissue labeling mouse CD31 antibody with IR75-253 at 1/200.
IR75-253 anti-mouse CD31 antibody FACS image
Mouse splenocytes were stained with IR75-253, mouse CD31 antibody