Product Includes
Application Dilution
Immunofluorescence 1:200 – 1:500
Immunohistochemistry (Paraffin) 1:200 – 1:300
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
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Reviews
IR1-2-2 anti-Vinculin antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR1-2-2 anti-Vinculin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR1-2-2 anti-Vinculin antibody WB image
All lanes : Anti-Vinculin antibody - Loading Control at 1/3000 dilution
Lysates/proteins at 30 μg per lane
This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR1-2-2 overnight at 4°C.
IR1-2-2 anti-Vinculin antibody WB image
All lanes : Anti-Vinculin antibody - Loading Control at 1/3000 dilution
Lysates/proteins at 30 μg per lane
This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR1-2-2 overnight at 4°C.
IR1-2-2 anti-Vinculin antibody IHC image
Immunohistochemical analysis of
paraffin embedded mouse testis
labeling Vinculin with IR1-2-2 at 1/300.