- Real Femto Plus Western Substrate based substrates can detect horseradish peroxdase at the femtogram level. Real Femto Plus Western Substrate provides superior sensitivity compared to competitor products . The substrate is supplied as two components.
| Real Femto Plus | Content | Package |
|---|---|---|
| IRRG8001-20ML | Real Femto Plus Western Substrate Solution A Real Femto Plus Western Substrate Solution B |
10ml 10ml |
| IRRG8001-50ML | Real Femto Plus Western Substrate Solution A
Real Femto Plus Western Substrate Solution B |
25ml 25ml |
| IRRG8001-100ML | Real Femto Plus Western Substrate Solution A
Real Femto Plus Western Substrate Solution B |
50ml 50ml |
| IRRG8001-200ML | Real Femto Plus Western Substrate Solution A Real Femto Plus Western Substrate Solution B |
100ml 100m |
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Store the reagents , solution-A and solution-B, at 2-8°C.
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Mix equal volumes of solution-A and solution-B in a clean container and mix well at room temperature before use.
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HRP enzyme substrate has a wide range to detect HRP enzyme on a membrane. Lower enzyme detection is at the femtogram level and the highest concentration of HRP enzyme detection may be one nanogram depending on the source of the HRP enzyme.
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Do not contaminate the HRP enzyme substrate with HRP enzyme or other proteins.
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For good results , wash your tube , microtiter plate or membrane with 0.2M phosphate buffer, pH 8.4 to 8.6 and then use the substrate. 6. Best results for chemiluminescence can be obtained from 5 minutes to 30 minutes after contacting substrate with HRP enzyme.