Product Includes
Application Dilution
Immunofluorescence 1:200-1:500
Immunohistochemistry (Paraffin) 1:50-1:100
Storage
100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
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Reviews
IR23-P1 anti- Neurofilament-L / NF-L antibody WB image
Anti- Neurofilament-L / NF-L antibody at 1/1000 dilution. Lysates at 40 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with IR23-P1 overnight at 4°C
IR23-P1 anti- Neurofilament-L / NF-L antibody IF image
Immunofluorescent analysis.
Sample: primary cortical neurons
NF-L antibody (IR23-P1): 1-250
Red: Tuj1 antibody
Anti-rabbit 488: 1-500
Fixed: 4% paraformaldehyde at RT for 20 min.
IR23-P1 anti- Neurofilament-L / NF-L antibody IHC image
Immunohistochemical analysis of paraffin embedded Human brain tissue labeling Neurofilament-L / NF-L with IR23-P1 at 1/100.