Product Includes
Application Dilution
Immunofluorescence 1:200-1:500
Storage
100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
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Atalog Number : IR23-P2
Dilution : 1:200
Reactivity : immunofluorescent
Sample : rat penis
Red : NF-L-P2 antibody
Anti-rabbit 594 : 1-400
Fixed: 4% paraformaldehyde at RT for 50 min
IR23-P2 anti- Neurofilament-L / NF-L antibody WB image
All lanes : Anti- Neurofilament-L / NF-L antibody at 1/1000 dilution
Lysates/proteins at 60 μg per lane
This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with IR23-P2 overnight at 4°C.
IR23-P2 anti- Neurofilament-L / NF-L antibody IF image
Immunofluorescent analysis.
Sample: primary cortical neurons
NF-L antibody (IR23-P2): 1-250
Red: Tuj1 antibody
Anti-rabbit 488: 1-500
Fixed: 4% paraformaldehyde at RT for 20 min.