Product Includes
Application Dilution
Immunofluorescence 1:200–1:500
Immunohistochemistry (Paraffin) 1:100–1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300.
Specificity / Sensitivity
Source / Immunogen
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]
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Application : Western blot
Condition / Dilution： 1-1000
Sample : HCT-116 drug treatment
Data from : Taipei Medical University
IR48-138 anti-Fibronectin antibody WB image
Anti-Fibronectin antibody at 1/1000 dilution. Lysates at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was incubated with IR48-138 overnight at 4°C.
IR48-138 anti-Fibronectin antibody _Human Hepatocellular Carcinoma (HCC) IHC image
Immunohistochemical analysis of paraffin embedded human hepatocellular carcinoma (HCC) labeling Fibronectin with IR48-138 at 1/100.
IR48-138 anti-Fibronectin antibody _Human Hepatocellular Carcinoma (HCC) IHC image
Immunohistochemical analysis of paraffin embedded human hepatocellular carcinoma (HCC) labeling Fibronectin with IR48-138 at 1/100.
IR48-138 anti-Fibronectin antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Fibronectin with IR48-138 at 1/100.
IR48-138 anti-Fibronectin antibody WB image
All lanes : Anti-Fibronectin antibody at 1/500 dilution. Lysates at 60 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3%BSA for an hour before being incubated with IR48-138 overnight at 4°C.
IR48-138 anti-Fibronectin antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR48-138 anti-Fibronectin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR48-138 anti-Fibronectin antibody_Human Breast Carcinoma IHC image
Immunohistochemical analysis of paraffin embedded human breast carcinoma labeling Fibronectin with IR48-138 at 1/100.
IR48-138 anti-Fibronectin antibody_Human Breast Carcinoma IHC image
Immunohistochemical analysis of paraffin embedded human breast carcinoma labeling Fibronectin with IR48-138 at 1/100.