Product Includes
Application Dilution
Immunofluorescence 1:200–1:500
Immunohistochemistry (Paraffin) 1:100-1:200
Storage
100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]
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Application : Western blot
Condition / Dilution： 1-1000
Sample : CaCO-2 drug treatment LPS 24hr
Data from : Southern Taiwan University of Science and Technology
IR56-184 anti-ZO-1 antibody WB image
All lanes : Anti-ZO-1 antibody - at 1/500 dilution
Lysates/proteins at 50 μg per lane.
This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with IR56-184 overnight at 4°C.
IR56-184 anti-ZO-1 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling ZO-1 with IR56-184 at 1/100.
IR56-184 anti-ZO-1 antibody WB image
All lanes : Anti-ZO-1 antibody - at 1/500 dilution
Lysates/proteins at 50 μg per lane.
This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with IR56-184 overnight at 4°C.
IR56-184 anti-ZO-1 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR56-184 anti- ZO-1 antibody (Green) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain
IR56-184 anti-ZO-1 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR56-184 anti- ZO-1 antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain.