Product Includes
Application Dilution
Immunofluorescence 1:100–1:200
Immunohistochemistry (Paraffin) 1:50-1:100
Storage
100mM Tris Glycine, 1%BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
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Application : WB
Condition : 1-1000
Data from : Kaohsiung Medical University
IR76-259 anti-CD34 antibody WB image
Anti-CD34 antibody - at 1/500 dilution. Lysates at 30 μg per lane.
This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with IR76-259 overnight at 4°C.
IR76-259 anti-CD34 antibody WB image
Anti-CD34 antibody - at 1/500 dilution. Lysates at 30 μg per lane.
This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with IR76-259 overnight at 4°C.
IR76-259 anti-CD34 antibody_Human placenta tissue IHC image
Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling CD34 with IR76-259 at 1/100.
IR76-259 anti-CD34 antibody_Kidney carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human kidney cancer tissue labeling CD34 with IR76-259 at 1/100.
IR76-259 anti-CD34 antibody_MDA-MB-231 ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR76-259 anti- CD34 antibody (red) at 1:100 and 4°C. DAPI (blue) was used as the nuclear counter stain.