Product Includes
Application Dilution
Immunofluorescence 1:200–1:500
Immunohistochemistry (Paraffin) 1:100–1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]
Reviews
IR79-272 anti-VCAM-1 / CD106 antibody WB image
VCAM-1 / CD106 antibody - at 1/1000 dilution
Lysates/proteins at 50 μg per lane
This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with IR79-272 overnight at 4°C.
IR79-272 anti-VCAM-1 / CD106 antibody_Breast carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling VCAM-1 / CD106 with IR79-272 at 1/100.
IR79-272 anti-VCAM-1 / CD106 antibody WB image
VCAM-1 / CD106 antibody - at 1/1000 dilution
Lysates/proteins at 50 μg per lane
This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with IR79-272 overnight at 4°C.
IR79-272 anti-VCAM-1 / CD106 antibody WB image
VCAM-1 / CD106 antibody - at 1/1000 dilution
Lysates/proteins at 50 μg per lane
This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with IR79-272 overnight at 4°C.
IR79-272 anti-VCAM-1_CD106 antibody ICC_IF image
Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling VCAM-1 / CD106 with IR79-272 at 1/100.
IR79-272 anti-VCAM-1 / CD106 antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR79-272 anti- VCAM-1 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR79-272 anti-VCAM-1 / CD106 antibody_Colorectal carcinoma IHC image
Immunohistochemical analysis of paraffin embedded Human colorectal cancer tissue labeling VCAM-1 / CD106 with IR79-272 at 1/100.