Product Includes
Application Dilution
Immunofluorescence 1:200 – 1:500
Immunohistochemistry (Paraffin) 1:100 – 1:200
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]
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Reviews
Customer feedback Image
Application : Western blot
Condition / Dilution： 1-1000
Sample : Raw264.7 / LPS treatment
Data from : Yuanpei University of Medical Technology
Customer feedback Image
Application : FFPE immunohistochemistry
Condition / Dilution：1-200
Epitope Retrieval methods: Citrate Buffer, pH 6.0
Sample : mouse liver
Data from : Li-Tzung Biotechnology. Co
Customer feedback Image
Application : Western blot
Condition / Dilution ：1-1000
Sample : SH-SY5Y drug treatment
Data from : Chang Gung University
IR109-446 anti-COX2 antibody WB image
Anti-COX2 antibody - at 1/1000 dilution
Lysates at 35 μg per lane. This blot was produced using a 7.5% SDS-PAGE. NC membrane was then blocked for an hour before being incubated with IR109-446 overnight at 4°C.
IR109-446 anti-COX2 antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse tissue labeling COX2 with IR109-446 at 1/100
IR109-446 anti-COX2 antibody IHC image
Immunohistochemical analysis of paraffin embedded mouse tissue labeling COX2 with IR109-446 at 1/100
IR109-446 anti-COX2 antibody ICC/IF image
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with IR109-446 anti-COX2 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR109-446 anti-COX2 antibody IHC image
Immunohistochemical analysis of paraffin embedded human cancer tissue labeling COX2 with IR109-446 at 1/100