Product Includes
Application Dilution
Immunofluorescence 1:100-1:300
Immunohistochemistry (Paraffin) 1:100-1:150
Storage
100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]
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Application : Western blot
Condition / Dilution： 1-1000 RT 2hr
Sample : Neuro2a
Data from : Taipei Medical University
IR101-420 anti-PARP cleaved antibody WB image
Anti-PARP celaved antibody - at 1/500 dilution. Lysates/proteins at 40 μg per lane.
This blot was produced using a 5% and 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane was then blocked an hour before being incubated with IR101-420 overnight at 4°C.
IR101-420 anti-PARP cleaved antibody_HCT-116 WB image
Anti-PARP celaved antibody - at 1/500 dilution. Lysates/proteins at 40 μg per lane.
This blot was produced using a 5% and 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane was then blocked an hour before being incubated with IR101-420 overnight at 4°C.
IR101-420 anti-PARP cleaved antibody_RAW264.7 WB image
Anti-PARP celaved antibody - at 1/500 dilution. Lysates/proteins at 40 μg per lane.
This blot was produced using a 5% and 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane was then blocked an hour before being incubated with IR101-420 overnight at 4°C.
IR101-420 anti-PARP cleaved antibody_293T WB image
Anti-PARP celaved antibody - at 1/500 dilution. Lysates/proteins at 40 μg per lane.
This blot was produced using a 5% and 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane was then blocked an hour before being incubated with IR101-420 overnight at 4°C.
IR101-420 anti-PARP cleaved antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR101-420 anti- PARP cleaved antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
IR101-420 anti-PARP cleaved antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PARP1 cleaved with IR101-420 at 1/100.
IR101-420 anti-PARP cleaved antibody IHC image
Immunohistochemical analysis of paraffin embedded Human oral carcinoma labeling PARP1 cleaved with IR101-420 at 1/150.