Product Includes
Application Dilution
Immunofluorescence 1:500 – 1:1000
Immunohistochemistry (Paraffin) 1:400 – 1:600
Storage
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Specificity / Sensitivity
Source / Immunogen
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
IR31-90 anti-NSE / Enolase-2 antibody WB image
Anti-NSE / Enolase-2 antibody at 1/3000 dilution Lysates at 30 μg per lane.
This blot was produced using a 10% SDS-PAGE.
The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane was then blocked for an hour before being incubated with IR31-90 overnight at 4°C.
IR31-90 anti- NSE / Enolase-2 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human brain tissue labeling NSE / Enolase-2 with IR31-90 at 1/400.
IR31-90 anti- NSE / Enolase-2 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human brain tissue labeling NSE / Enolase-2 with IR31-90 at 1/400.
IR31-90 anti-Enolase-2 / ENO2 antibody IHC image
Immunohistochemistry (Frozen sections)
Sample: mouse retina tissue sections
Antibody: Enolase-2 / ENO2 antibody (IR31-90) at 1/500 dilution
IR31-90 anti- NSE / Enolase-2 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human prostate cancer tissue labeling NSE / Enolase-2 with IR31-90 at 1/400.
IR31-90 anti- NSE / Enolase-2 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue labeling NSE / Enolase-2 with IR31-90 at 1/400.
IR31-90 anti- NSE / Enolase-2 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human brain tissue labeling NSE / Enolase-2 with IR31-90 at 1/400.
IR31-90 anti- NSE / Enolase-2 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue labeling NSE / Enolase-2 with IR31-90 at 1/400.
IR31-90 anti- NSE / Enolase-2 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human brain tissue labeling NSE / Enolase-2 with IR31-90 at 1/400.
IR31-90 anti- NSE / Enolase-2 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human lung cancer tissue labeling NSE / Enolase-2 with IR31-90 at 1/400.
IR31-90 anti- NSE / Enolase-2 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human Tonsillar malignancy tissue labeling NSE / Enolase-2 with IR31-90 at 1/400.
IR31-90 anti- NSE / Enolase-2 antibody IHC image
Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling NSE / Enolase-2 with IR31-90 at 1/400.