Product Includes
Application Dilution
Immunofluorescence 1:100 – 1:300
Immunohistochemistry (Paraffin) 1:100 – 1:150
Storage
100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Specificity / Sensitivity
Source / Immunogen
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]
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Reactivity Human
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Reactivity Human
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Reactivity Mice
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Reactivity Human
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Reviews
Customer feedback Image
Application : Western blot
Condition / Dilution： 1-1000, BSA blocking
Sample : HCT-116 drug treatment
Data from : Taipei Medical University
IR101-421 anti-PARP cleaved antibody WB image
All lanes : Anti-PARP1 antibody - 1/1000 dilution. Lysates at 40 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with IR101-421 overnight at 4°C.
IR101-421 anti-PARP antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PARP1 cleaved with IR101-421 at 1/200.
IR101-421 anti-PARP antibody IHC image
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PARP1 cleaved with IR101-421 at 1/200.
IR101-421 anti-PARP antibody ICC/IF image
Immunofluorescence: cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with IR101-421 anti- PARP cleaved antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.