![IR31M53 anti-NSE / Enolase-2 monoclonal antibody [12F] WB image](/Upload/Products/1722570024_a80ba8a16c8a2a02438a.jpg "IR31M53 anti-NSE / Enolase-2 monoclonal antibody [12F] WB image")
![IR31M53 anti-NSE / Enolase-2 monoclonal antibody [12F] IHC image](/Upload/Products/1722570164_c5afd52c1f225ce494eb.jpg "IR31M53 anti-NSE / Enolase-2 monoclonal antibody [12F] IHC image")
Catalog Number：IR31M53
NSE / Enolase-2 monoclonal antibody [12F]
Application
Reactivity Hu、Ms、Rat
| REACTIVITY | Hu、Ms、Rat |
|---|---|
| SENSITIVITY | Endogenous |
| MW | 47 |
| SOURCE | Mouse IgG1 |
Product Includes
-
Application Dilution
Western Blot 1:1000– 1:12000
Immunofluorescence 1:500 – 1:1000
Immunohistochemistry (Paraffin) 1:200 – 1:400
Immunofluorescence 1:500 – 1:1000
Immunohistochemistry (Paraffin) 1:200 – 1:400
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C
Phosphate buffered solution, pH 7.2, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Phosphate buffered solution, pH 7.2, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Specificity / Sensitivity
Endogenous
Source / Immunogen
-
-
IR31M53 anti-NSE / Enolase-2 monoclonal antibody [12F] WB image
This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane was then blocked for an hour before being incubated with IR31M53 overnight at 4°C.
Anti-NSE / Enolase-2 monoclonal antibody [12F] at 1/1000 dilution.
Lysates at 30 μg per lane.
IR31M53 anti-NSE / Enolase-2 monoclonal antibody [12F] IHC image
Immunohistochemical analysis of paraffin embedded human breast cancer (TNBC) tissue labeling NSE / Enolase-2 with IR31M53 at 1/200.